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KMID : 1101320090410040158
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Korean Journal of Clinical Laboratory Science
2009 Volume.41 No. 4 p.158 ~ p.166
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Availability of Identification by RAPD of Aspergillus species from Sputum
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Kim Young-Kwon
Hong Sung-Rho
Kim Sang-Ha
Seo Choong-Won
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Abstract
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On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial β-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphology examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1) and Neosatorya fennelliae (1). This is the first report of A. tubuingensis in clinical field in Korea.
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KEYWORD
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Aspergillus, Tuberculosis, Sputum, RAPD, β-tubulin, Aspergillosis
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